Imaging Core
The HIHG Imaging Core offers UM colleagues and the South Florida scientific community a resource for microscopic imaging that is constantly updated and improved to remain cutting edge. Currently installed:
Equipped with four lasers: 1. 405nm diode laser: for the ultraviolet-violet excitation spectral range dyes (DAPI, Hoechst, BFP); The new Quasar spectral detector permits recording of X(YZT) lambda image stacks for the localized analysis of spectral signatures of the specimen and enables up to 10 emission signals to be precisely separated via software, even if the fluorescence spectra are overlapping. The ZEN 2009 software includes a spectral "Smart Setup" to help setup optimized image acquisition. Smart Setup uses the open data access PubSpectra dataset developed by Dr. McNamara, http://sylvester.org/AICF/PubSpectra. The LSM710 microscope includes motorized XY stage and Z focus, with both stage tiling and mark & find capability for Z-series and/or time-series. 37 Celsius warming units are available for both 35 mm imaging dishes (e.g. from www.glassbottomdishes.com, FluoroDishes from www.wpi-inc.com, Willco Wells from http://www.tedpella.com, CellView from www.greinerbioone.com) and for 24-well multiwell plates (e.g. Corning 3526 plastic bottom for low resolution imaging, P24G-1.5-13-F from www.glassbottomdishes.com for high resolution imaging). If your specimen is to be imaged at room temperature, you can use a microscope slide or a 35 mm imaging dish, 50 mm imaging dish, or any SBS multiwell plate format (6, 12, 24, 48, 96, 384). Best results are obtained from image-quality dishes or plates cited above. For multiwell plates, consider Whatman ClearView or glass bottom plates (http://www.whatman.com/SpecialtyMicroplates.aspx, BD Falcon glass bottom plates http://www.bd.com/resource.aspx?IDX=1108, or Aurora Biotechnologies COP high quality plastic plates http://www.aurorabiotechnologies.com. . Applications: high resolution cell and tissue imaging, Z-series, XY tile scanning, FRET, FRAP, time lapse live cell imaging with 37 C control, optional humidity, CO2 control. Additional Microscopes:
For more information, please contact George McNamara, Ph.D. gmcnamara@med.miami.edu
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