Imaging Core

The MIHG Imaging Core offers UM colleagues and the South Florida scientific community a resource for microscopic imaging that is constantly updated and improved to remain cutting edge.

Currently installed:

• Zeiss LSM 510 Meta confocal microscope.

Equipped with four lasers lines:

405nm diode laser: for the ultraviolet spectral range dyes;
458nm/477nm/488nm/514nm Argon lasers: for blue range spectral dyes;
561 diode laser: for the green spectral range dyes;
633 nm HeNe laser: for red spectral range dyes.

A meta detector permits recording of X(YZT) lambda image stacks for the localized analysis of spectral signatures of the specimen and enables up to eight emission signals to be precisely separated via software, even if the fluorescence spectra are overlapping.

Applications: FRAP, FRET, time lapse live cell imaging

• Nikon bright field Eclipse 50i microscope, equipped with Qimaging MicroPublisher 5.0   RTV digital digital camera.
• Nikon Eclipse TE2000-U fluorescent microscope equipped with Qimaging Retiga EXi  Fast 1394 digital camera.
• Leica DMIL inverted microscope for cell culture equipped with Qimaging QICAM Fast 1394 digital camera
  

Contact: Dr.Chunrong Lu at: clu@med.miami.edu